Banerjee A, Sengupta A, Maji B, Nandi A, Pal S and Mukherjee S
Background: Hepatocellular carcinoma (HCC) is the fifth most basic type of disease on the planet. Regardless of such a variety of choices for HCC treatment, the cure rate for patients is generally low especially among patients who are ineligible for surgical or percutaneous methods. HCC is generally viewed as a chemotherapy-safe malady. These disadvantages require the proceeded with scan for novel HCC treatments. The plant Annona muricata has been accounted for to have acetogenins as major phytoconstitutent which are in charge of number of exercises and one of them is anticancer and antiproliferative impacts against different tumors. The present study was intended to assess the anticancer potential of aqueous extract of Annona muricata leaves (AEAML) against Huh-7 human liver cancer cells.
Methods: The cytotoxic impact of AEAML on Huh-7 cells was tried by MTT examine. Cells were tested with 0.5, 1.0, and 1.5 mg/ml concentrate of AEAML for 24 hours. The apoptotic genes Bax and Bcl-2 expression were assessed by utilizing RT-PCR, and after that decide the protein expression of caspase 3 and 9 by western blot. Comet assay was performed in Huh-7 cells for the DNA damage and apoptosis.
Results: Cell viability investigation uncovered the cytotoxic impact of AEAML on Huh-7cell line. RT-PCR of anti- apoptotic Bcl-2 and pro-apoptotic Bax demonstrated that there is down and up control, individually, of these two qualities with an unfriendly impact. Additionally, AEAML treatment set off the actuation of caspase-9 followed by caspase-3 protein expression. Also, AEAML incited DNA damage and apoptosis on Huh-7 cells as uncovered by Comet tail length.
Conclusion: Annona muricata has antiproliferative and cytotoxic impacts on Huh-7 cells, conceivably through apoptosis induction.
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