John Andreus
The analytical methods of greening have gained rising interest in the field of pharmaceuticals to improve the health safety of analysts and to decrease environmental impacts. Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most widely used analytical technique in pharmaceutical drug development and manufacturing such as the quality control of bulk drugs and pharmaceutical formulations, along with the analysis of drugs in biological samples. However, RP-HPLC methods commonly use large amounts of organic solvents and generate high quantities of waste to be disposed of, leading to some issues in terms of ecological impact and operator safety. In these contents, greening HPLC methods is becoming highly desirable. One strategy to reduce the impact of hazardous solvents is to replace classically used organic solvents with greener ones. As yet, ethanol has been the most often used alternative organic solvent.
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