Yuanyuan Xu , Han Zhang , Weidong Chang , Li Wang and Linsong Wan
The abnormal expression of miR-193a-3p was associated mostly with the development and progress of the tumors. In the present study, the expression levels of miR-193b-3p and its associated mRNAs were measured by reverse transcription-quantitative polymerase chain reaction, and the methylation levels of its promoter were detected via the methylation-specific PCR in serum and tissues of patients with esophageal squamous cell carcinoma (ESCC). The results demonstrated that the expression level of miR-193b-3p were significantly decreased in preoperative serum and cancer tissues in patients with ESCC (p<0.01). The expression level of miR-193b-3p significantly was upregulated in serum after surgical removal of the cancerous tissue in ESCC patients (p<0.01). The target gene CCND1 of miR193a-3p showed the high expression in the tumor tissues, and its up- and down-stream gene Rb and p16 showed the low expression; and the target gene IGF1R and its up- and down-stream gene IGF1 and PI3K showed the high expression. The methylation level of miR-193a-3p in the promoter region was higher significantly in the tumor tissues than that in the adjacent non-cancerous tissues (p<0.01). This showed that the high methylation of genetic promoter region of miR-193b-3p might inhibit the expression of miR-193b-3p and further the regulation of CCND1 and IGF1R, as well as the relevant signaling pathways, facilitating the development and progress of ESCC.
इस लेख का हिस्सा
English 
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Telugu 