Manickam Raja and Pachiappan Perumal
DNA barcoding is an effective tool for the identification of species representing diverse taxa especially through the sequence analysis of mitochondrial cytochrome c oxidase subunit I (COI) gene. In the present study, DNA barcodes were generated from 46 species of freshwater fishes covering the Orders Cypriniformes, Siluriformes, Synbranchiformes and Perciformes representing 30 genera under 9 families. All the samples were collected from diverse sites which also includes some endemic species. A total of 47 COI sequences were generated. After amplification and sequencing of 678 base pair fragment of COI, primers were trimmed which invariably generated a 635 base pair barcode sequence. The average Kimura two-parameter (K2P) distances within-species, genera, families, and orders were 0.32%, 8.40%, 14.50%, and 18.65%, respectively. DNA barcode discriminated congeneric species without any confusion. The present study strongly supports the efficiency of COI as an ideal marker for DNA barcoding of selected freshwater fishes.
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