बायोप्रोसेसिंग और बायोटेक्निक्स जर्नल

The current work deals with the studies of isolation and preliminary characterization of the bacteria isolated from higher termite soil. Termites play an important role in the turnover and mineralization of complex biopolymers, such as wood and other cellulose and hemicelluloses containing materials. Lignocellulose is the most predominant component of the woody and dead plant materials, as well as it is the most abundant biomass on earth, especially in terrestrial ecosystems. Thus the work focuses on using lingo cellulosic waste efficiently for production of Carboxy Methyl Cellulase (CMC). As CMCase have higher hydrolyzing capacity than the other two cellulases, cellobiase and cellobiohydralases it has wide application in industry such as bread and baking industry, laundry etc. Thus the work focuses on identification of efficient bacterial species in this work 6 bacterial species were isolated among which I4 produced higher CMCase enzyme. After the morphological, biochemical and identification test, the I4 isolate was found to be Bacillus sp.

Sugarcane fibres, in the form of bagasse, are abundant by-products from the sucro-alcohol industry and are commonly used as reinforcement in composite materials. The main objective of this work concerns the modification of the sugarcane bagasse fibers (in natura and pulps rich in cellulose) by benzoylation. Unfortunately, the natural fibres have hygroscopic properties, causing poor fibre/matrix interaction. In order to encourage non-polar properties in the fibres, this current work is concerned with the feasibility of aromatic group insertion in natural fibre structures using benzoylation reactions. Sugarcane fibres were soaked in aqueous NaOH and mixed with benzoyl chloride. After chemical treatments, the modified sugarcane fibres were characterized by weight gain, Fourier Transformer Infrared-FTIR, Scanning Electron Microscopy SEM, thermal analysis, mechanical tests and statistical analysis. Results show that there was significant weight gain during the first hour of the reaction. Esterification reaction efficiencies were confirmed by FTIR. SEM characterization revealed that the chemical treatment changed the morphology of the fibres. Thermogravimetric-TG curves illustrated different behaviour between modified and nonmodified fibres. Mechanical properties show that the benzoylated fibres insertion caused an increase in the tensile strength in regard to non-modified fibres. Finally, the results for strength obtained from the uniaxial tensile tests demonstrated the effectiveness of treating the fibres with benzoyl groups, with condition C6 providing the best results, followed by condition C7.