रासायनिक विज्ञान जर्नल

Identification of Metabolites using 1H NMR in Polycystic Ovary Syndrome Cases from North India

Abstract

Ratneev Kaur , Tajinder Kaur , Palwinder Singh and Anupam Kaur

Polycystic Ovary Syndrome (PCOS) is a common, heterogenous endocrine disorder in women of reproductive age. Alterations in numerous metabolic pathways have been implicated in its pathophysiology. The systemic changes in PCOS initate not only changes in the ovarian function but also changes in the whole-body metabolism. Indian women are at higher risk of developing type 2 diabetes and insulin resistance and hence give the drive to carry metabolomics study on Indian women. The present study focused on the metabolic profiling of PCOS women in North India. A total of 28 females of reproductive age were included. 1HNMR technique was used to identify metabolites. Orthogonal projection to the latent structure with discriminant analysis (OPLS-DA) was applied for model identification. Important metabolites were identified using variance of importance (VIP) score and s-plot and the significance was verified by univariate analysis. The levels of leucine, glutamine, citric acid, proline and, methionine were decreased and that of valine, tyrosine and phenylalanine were elevated in PCOS women as compared to control females. The differences in the metabolic profiles between PCOS cases and controls may be attributable to the alterations in various pathways. The present study is first of its kind from North India.