मेडिकल माइक्रोबायोलॉजी और निदान

Journal of Medical Microbiology and Diagnosis commemorates its decade long service to the scientific community by consistently publishing peer-reviewed articles and tracking the progress and significant advancements in the field of Microbiology. Ever since its inception in the year 2012, in addition to regular issue releases on a quarterly basis, this transdisciplinary journal is also releasing special issues and conference proceedings from time to time, thus comprehensively covering a wide range of topics and emerging challenges in Bacteriology, Clinical and Medical Diagnostics, Parasitology, Bacterial Infections. The journal focuses on application oriented research on Bacteriology, Clinical and Medical Diagnostics, Parasitology, Bacterial Infections. In this issue some of the recent and impactful research articles that were published by the journal will be discussed.

Coronavirus disease (COVID-19) is caused by SARS-COV2 and represents the causative agent of a potentially fatal disease that is of great global public health concern. Based on the large number of infected people that were exposed to the wet animal market in Wuhan City, China, it is suggested that this is likely the zoonotic origin of COVID-19. Person-to-person transmission of COVID-19 infection led to the isolation of patients that were subsequently administered a variety of treatments. Extensive measures to reduce person-to-person transmission of COVID-19 have been implemented to control the current outbreak. Special attention and efforts to protect or reduce transmission should be applied in susceptible populations including children, health care providers, and elderly people. In this review, we highlights the symptoms, epidemiology, transmission, pathogenesis, phylogenetic analysis and future directions to control the spread of this fatal disease.

This year, the Journal of Medical Microbiology and Diagnosis commemorates its decade long publication service in the field of Microbiology. With a successful International publications record, the journal has already begun compilation of the third issue in the 9th volume. In addition to the regular issues the journal has been publishing special issues, supplements and conference proceedings from time to time. In general, the journal covers all including Bacteriology, Clinical and Medical Diagnostics, Parasitology, Bacterial Infections. With good citation record, the journal is included in indexing databases conferring wide geographical outreach, such as Index Copernicus, Open J Gate, CAS Source Index along with simultaneous portproduction digital media promotion policy. Ever since its inception in the year 2012 the journal has been consistently producing quality articles sourced from all across the world. The journal holds impeccable record of regular bimonthly issue release frequency with publication time lines. 

Journal of Medical Microbiology and Diagnosis commemorates its decade long service to the scientific community by consistently publishing peerreviewed articles and tracking the progress and significant advancements in the field of Microbiology. Ever since its inception in the year 2012, in addition to regular issue releases on a quarterly basis, this transdisciplinary journal is also releasing special issues and conference proceedings from time to time, thus comprehensively covering a wide range of topics and emerging challenges in Bacteriology, Clinical and Medical Diagnostics, Parasitology, Bacterial Infections. The journal focuses on application oriented research on Bacteriology, Clinical and Medical Diagnostics, Parasitology, Bacterial Infections. In this issue some of the recent and impactful research articles that were published by the journal will be discussed. 

Acinetobacter baumannii is a nosocomial pathogen which the World Health Organization̕ s considered number one critical priority pathogen. It has become a growing problem in hospitals as a predominant multi-drug resistant that left clinicians with limited treatment options. Its main mechanisms for β-lactam resistance are the production of carbapenems especially Amber class D β-lactamases followed by B β-lactamases. NDM-1 which is an example of the later poses a major health concern particularly in the light of its spread through population. To precede our study, 74 Acinetobacter baumannii isolates were collected from hospital laboratories during the period from July 2017 till June 2018. Antimicrobial susceptibility testing was done by Kirby-Bauer Disc Diffusion method (KBDD) and Minimum Inhibitory Concentration (MIC) was detected using E-test method. CRAB ones were tested by both of Modified Hodge Test (MHT) and Imipenem EDTA Combined Disc Test (CDT) to detect metallo βeta-lactams (MBL) producers. Then existence of NDM-1 gene was further identified. All of the 74 Acinetobacter baumannii isolates were found to be multi-drug resistant (MDR). 36 of them (48.65%) were resulted as carbapenem resistant and 27 (36.49%) were metallo βeta-lactams (MBL) producers. 12/27 (44.44%) MBL poses NDM-1 gene in the first detection procedure while the other negative 15 ones the percentage of NDM-1 was found to be 10/15 (66.67%) when using different primer. PCR products were then verified by DNA sequencing. The final consensus sequences were analyzed and submitted to NCBI GenBank data base, representing accession numbers are JF838352.1, MK682768.1 and MN251667.1. The alignments showed similarity ranged from 94%-96.4% amino acids identity. We concluded that detection of antibiotic resistant Acinetobacter baumannii revealed that multi-drug resistance arises dramatically due to the indiscriminate use of antibiotics and the poor applying of infection control precautions. These results emphasize the importance of implementation and comply both of antibiotic and infection control policies.