Qing Zhang*
Following the anaerobic fermentation process in a lagoon, the effluent from pig wastewater exhibited elevated levels of total nitrogen and nitrate nitrogen. The utilization of simple sulphur-limestone autotrophic denitrification resulted in a sluggish reaction rate and relatively moderate removal load. To enhance the efficacy of the sulphur autotrophic denitrification process, a strategy was implemented involving the introduction of a limited quantity of corncobs as a slow-release carbon source. This addition was made to a sulphur-limestone autotrophic denitrification reactor that was being operated in a continuous and stable manner. Furthermore, the volume ratio of the corncobs added was progressively increased. This study examines the alterations in nitrate nitrogen, Chemical Oxygen Demand (COD), sulphate, and alkali consumption within the sulphur-limestone autotrophic denitrification reactor, both prior to and after to the introduction of corncobs. This study examines the operational efficiency and reaction mechanism of a sulfur-limestone autotrophic denitrification reactor that is enhanced by the utilization of corncobs as a slow-release carbon source. The findings of the study indicate that the inclusion of maize cob carbon source in the sulphur limestone autotrophic denitrification process resulted in an improved effectiveness in removing nitrate and nitrogen. Additionally, when considering the three different ratios of corn cob carbon source, the process exhibited a Chemical Oxygen Demand (COD) removal rate exceeding 42%. The observed trend in the consumption of alkalinity in both the pure sulphur limestone autotrophic denitrification process and the sulphur limestone autotrophic denitrification process augmented by the three types of corncob slow-release carbon sources indicated an increase with time. The concentration of nitrate-nitrogen in the influent water exhibited a negative correlation with the length of the reactor flow-through. The addition of a slow-release carbon source derived from corncob greatly enhanced the autotrophic denitrification and denitrification impact in the three reactors. Moreover, there was an observed increase in the trend of heterotrophic denitrification as the amount of carbon source added increased. The experiments investigating mixotrophic denitrification reaction observed a positive correlation between the content of corn cob and the relative abundance of microbial flora. Specifically, an increase in corn cob content led to an increase in the relative abundance of Proteobacteria at the phylum level. Additionally, the relative abundance of various other flora at the genus level also increased proportionally. The efficacy of the mixotrophic denitrification process surpassed that of the sulphur-limestone autotrophic denitrification method in the treatment of nitrate-nitrogen wastewater characterized by a high concentration of nitrate-nitrogen. The former exhibited a higher denitrification rate and resulted in lower levels of sulphate ions and alkalinity production.
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