पशु चिकित्सा विज्ञान एवं प्रौद्योगिकी

Muscovy Duck Parvovirus Infection with Epicarditis in Bali, Indonesia

Abstract

Gusti Ngurah Mahardika, Made Bagus Arya Permana Ardiana Putra, Ni Putu Sutrisna Dewi, Ni Made Ritha Krisna Dewi and Ida Bagus Oka Winaya

Muscovy duck parvovirus (MDPV) is threatening industry and backyard Muscovy duck poultry throughout the world. Here, we confirmed its presence in Indonesia for the first time. The outbreak described in this study occurred in a small fatty liver (French: foie gras) industry in Tabanan, Bali, and affected ducklings aged 2–3 weeks. Although older Muscovy ducks were present at the facility, they did not present signs of illness. Among the ducklings, the morbidity and mortality rates were 100% and 90%, respectively. The initial incidence began on 15 June 2014 and ended a month later. It reoccurred in January 2015. Clinical signs were lethargy, anorexia, watery diarrhoea, and dyspnea. The most frequent pathological lesions were cardiac enlargement with pale pericardia, haemorrhage, and enlargement of the liver. Dominant histopathology features were severe enteritis, epicarditis, and hepatitis. Using polymerase chain reaction (PCR) and published primer pairs NS1/REP and VP1, MDPV infection was confirmed with whole DNA isolated from the heart and liver homogenates. Sequencing of the PCR products resulted in 900 bp NS1/REP and 1200 bp VP1 fragments specific to MDPV. The virus sequences from the two separated incidences were completely homologous with one another. Now that MDPV has been detected in Indonesia, it should be included in the differential diagnosis when evaluating ducklings with MDPV-associated symptoms.