Jane F Turton, Claire Perry and Matthew J Hannah
We successfully isolated bacteriophage active against clinical strains of Acinetobacter baumannii by enrichichment from activated sewage sludge using representatives of those strains. Purified bacteriophage suspensions obtained were tested on a range of clinical isolates that included representatives of multiple strains of each of the international clonal lineages, as well as minor and sporadic strains. Most representatives of the South East clone, a successful sublineage belonging to international clone II, and a representative of a further strain belonging to international clone II were susceptible to the phage, while further strains belonging to this lineage, as well as those from other lineages were not susceptible. Examination by transmission electron microscopy revealed bacteriophage of the Myoviridae family. Activated sewage sludge is a valuable source of bacteriophages active against some currently circulating strains of A. baumannii.
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