जर्नल ऑफ माइक्रोबियल पैथोजेनेसिस

Identification and Antimicrobial Resistance Profile of Major Gastrointestinal Bacteria in Monkey (Olive Baboon)

Abstract

Khalid Z Rasib, Saniya yaqoob, Syed Zeeshan Haider1, Kiran Saleem, Faiza Pervaiz

All higher animals are associated with a diverse microbial community, mainly composed of bacteria. Diversity of gut micro-biota and health depends upon the food and environment. Zoo provides unintentionally diverse population exposure of microbes to animals as well as humans. About 60% of all human diseases and approximately 75% of emerging infectious diseases are zoonotic. Fecal samples were collected of Olive Baboon kept at Lahore zoo. Samples were collected by using sterilized polythene bags and shifted to the laboratory of Microbiology of IMBB Department of University of Lahore immediately. Primary isolation was done on Nutrient agar and resulting growth was shifted on Differential media by streaking method. Gram staining was performed for the confirmation of bacteria. Pure colonies of bacteria were isolated through selective media. Different biochemical tests were performed for the further confirmation of genus of bacteria. Different bacteria such as E. coli, Staphylococcus aureus, Shigella, Klebsiella spp., and Bacillus cereus were isolated. Antimicrobial resistivity of isolated bacteria was checked by using different antimicrobial discs and the zone of inhibition was measured in mm.