कैंसर विज्ञान एवं चिकित्सा

Effect of Fermented Soy Beverage Haelan 951 on the Viability of Human Cancer Cell Lines in vitro

Abstract

Ioanna Vlachou, Panagiotis Parsonidis and Ioannis Papasotiriou

The objective of this study is the assessment of the cytotoxic effect of a commercially available fermented soy beverage Haelan 951, which is a supplement widely used by cancer patients. The main ingredient of the product is soy that is rich in flavonoids, especially isoflavones. The formulation was tested for its efficacy as well as the determination of main ingredients with proliferation, viability, apoptosis assays and analytical techniques. Low concentrations of genistein and daidzein present in Haelan 951 were confirmed with HPLC, LC-MS and NMR. Cytotoxic effect against MCF7 cell line was observed with MTT, SRB and CVE at the maximum concentration (32%) after 48h of incubation. 8% Haelan 951 had apoptotic effect on all cancer cell lines tested. The formulation of the product could lead to false positive results as it was slightly soluble in all the solvents used and the viscosity was visually observed to be quite high.