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Chromatographic Method to Determine the Sorption of the Antibiotic Oxytetracycline to Tropical Soils

Abstract

Sílvio Vaz Jr

Abstract This analytical method was adapted to determine the sorption of oxytetracycline (OTC), an antibiotic of large use for animal and agriculture, to tropical soils. Tetracycline antibiotics for veterinary are considered emerging environmental contaminants. The high performance liquid chromatography (HPLC) with UV-visible detector in reverse mode was used to generate quantitative data that allowed the construction of sorption Freundlich isotherms for soils-OTC at pH 4.8. The Freundlich isotherm was presented as a mathematical model suitable for the verification of soils-OTC sorption from chromatographic data, proving the sorption and its type, as well as the soil-OTC sorption capacity. The method consists of: • Measuring the presence of the oxytetracycline (OTC) veterinary in tropical soils • Constructing Freundlich isotherm for sorption soil-OTC to understand the OTC’s fate on environment

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