Nazim Hussain, Lijie Xuan, Xinze Zhao and Lixi Jiang
To change the ratio of α-/γ-Tocopherol (Toc) is an important goal for rapeseed breeding. However, evidence showing the association between rapeseed VTE4 and seed Toc composition in oilseed crops is still insufficient. Single nucleotide polymorphism is a kind of molecular marker that causes phenotypic variations of a certain trait. We applied High-resolution Melting curve (HRM) technique to detect the single nucleotide polymorphisms (SNPs) at BnaX.VTE4.b in an ethyl methane sulfonate (EMS) mutated M2 population of rapeseed. There were remarkable variations among 1011 individual M2 plants in total Toc and Toc composition (α-/γ-Toc ratio). The total Toc varied in a range of 150.33–424.18 mg/kg with the mean of 293.39 (SD ± 4.23). The-/γ-Toc ratio varied in the range of 0.35-2.84 with the mean of 0.95 (SD ± 0.37). The association between detected SNPs and phenotypic variations in Toc composition was analyzed. The results revealed significant association between BnaX.VTE4.b and α-/γ-Toc ratio. Frequency of SNPs varied greatly among different regions of the gene and we found nonsynonymous SNPs for both high and low α-/γ-Toc ratios near 5?Š?¹ end of BnaX.VTE4.b. Moreover, linkage disequilibrium and association analysis revealed significant association between Toc composition and various SNPs on BnaX.VTE4.b providing useful markers for rapeseed quality breeding.
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