Yu-Jen Lin, Chia-Ying Wu, Taiwei Li, Pei-Wen Hsiao and Ding-Kwo Chang
We have developed a novel, rapid, highly sensitive biosensor protocol for diagnosis and characterization of influenza viruses in the early stage of the epidemic. Using Surface-Enhanced Raman Scattering (SERS) technique and nitrocellulose membrane, detection limit as low as 30 ng/mL was achieved with high selectivity to different subtypes of influenza virus tested. Moreover, the SERS measurement can be completed in less than 2 h, thus making the protocol useful in early diagnosis and impediment of propagation of potential influenza pandemic.
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